From Laboratory to Mass Production: The Complete Greenhouse Acclimatization Process for Phalaenopsis Tissue Culture Seedlings
Phalaenopsis, as a high-value ornamental flower, relies on the precise integration of tissue culture technology and greenhouse acclimatization for large-scale production. From the sterile environment of the plant tissue culture room to adaptation under natural greenhouse conditions, Phalaenopsis tissue culture seedlings undergo a series of environmental adjustments and physiological adaptations. This article details the complete greenhouse acclimatization process for Phalaenopsis tissue culture seedlings, with a focus on the roles of key facilities such as whole house circulation fans, dehumidification equipment, and heavy duty greenhouse frames.
1. Pre-Transfer Preparation for Tissue Culture Seedlings
Phalaenopsis tissue culture is typically conducted in a plant tissue culture room, where light, temperature, and humidity are strictly controlled to ensure the healthy growth of sterile seedlings. After months of proliferation and root development in culture bottles, the seedlings must undergo acclimatization before being transferred. The key to this stage lies in gradually adjusting environmental parameters to prepare the seedlings for external conditions.
1-2 weeks before transfer, the humidity in the culture room should be slightly reduced, and light intensity increased to simulate greenhouse conditions. Simultaneously, greenhouse facilities—particularly the stability of the heavy duty greenhouse frame and the operation of whole house circulation fans—should be inspected to ensure uniform air circulation.
2. Seedling Transfer and Greenhouse Acclimatization
(1) Selection of Transfer Substrate
The roots of Phalaenopsis tissue culture seedlings are delicate, requiring a well-aerated substrate such as sphagnum moss, bark, or coconut husk chips. The substrate must be sterilized before use to prevent pathogen infection.
(2) Environmental Control
The first two weeks post-transfer are critical for acclimatization, requiring precise control of temperature and humidity in the greenhouse:
Temperature: Maintain 25–28°C during the day and no lower than 20°C at night to avoid stress from sudden fluctuations.
Humidity: Initially maintain 80–90%, then reduce by 5% weekly until stabilizing at 60–70%. Dehumidification equipment plays a vital role in preventing fungal diseases caused by excessive humidity.
Light: Initial light intensity should be 5,000–8,000 lux to avoid leaf scorching, gradually increasing to 10,000–15,000 lux.
(3) Air Circulation Management
Proper air flow reduces disease incidence and enhances transpiration, promoting seedling adaptation. Whole house circulation fans ensure even air distribution, preventing localized high humidity or CO₂ deficiency. Fans should operate intermittently to avoid seedling dehydration from constant strong airflow.
3. Impact of Greenhouse Structure on Acclimatization
Modern Phalaenopsis production greenhouses often utilize heavy duty greenhouse frames, offering advantages such as:
High load-bearing capacity: Supports multi-tier cultivation racks, maximizing space efficiency.
Wind and pressure resistance: Ensures structural stability during extreme weather, protecting seedlings.
Integrated equipment compatibility: Facilitates installation of supplemental lighting, shading nets, and dehumidification equipment for precise environmental control.
Additionally, the choice of greenhouse covering materials (e.g., double-layered polycarbonate panels or diffuse glass) affects light uniformity, influencing Phalaenopsis growth consistency.
4. Pest and Disease Control During Acclimatization
High humidity environments are prone to fungal and bacterial diseases like soft rot and anthracnose. Preventive measures include:
Regularly inspecting dehumidification equipment to maintain optimal humidity levels.
Enhancing ventilation with whole house circulation fans to minimize leaf condensation.
Applying biopesticides or low-toxicity chemical sprays preventively.
5. Completion of Acclimatization and Subsequent Management
After 4–6 weeks of greenhouse acclimatization, Phalaenopsis seedlings develop thicker leaves and robust roots, adapting to standard cultivation conditions. Subsequent management includes:
Adjusting fertilization to increase phosphorus and potassium ratios, promoting flower bud differentiation.
Optimizing whole house circulation fan operation, increasing airflow during high-temperature periods to reduce leaf temperature.
Regularly inspecting the stability of the heavy duty greenhouse frame, especially before typhoon or heavy rain seasons, to reinforce structural integrity.
Conclusion
The greenhouse acclimatization of Phalaenopsis tissue culture seedlings is a systematic process involving environmental control, facility management, and pest/disease prevention. The plant tissue culture room provides high-quality seedlings, while whole house circulation fans, dehumidification equipment, and heavy duty greenhouse frames collectively create an efficient acclimatization environment. Through scientific process management, high survival rates and premium quality can be achieved, laying a solid foundation for mass production.
